Lastly, the reason a green light was used in order to see was because chlorophyll absorbs all colors of light except for green and yellow (chiras, 1993) additional experiments using different colors of light or different light bulbs would be interesting, as the chlorophyll trap different kinds of lights at different rates. If the spinach leaves are placed under blue or red light, the rate of photosynthesis will be faster than green light because chlorophyll a and b as well as several types of carotenoids are present in the spinach this causes an absorption of energy from red and blue wavelengths which is used in photosynthesis. Analysis of the absorption of green light versus red light absorption in spinach leaves the goal of the experiment was to determine if green light had less ability to absorb than red light in spinach leaves.
A) green and yellow wavelengths inhibit the absorption of red and blue wavelengths b) oxygen given off during photosynthesis interferes with the absorption of light c) other pigments absorb light in addition to chlorophyll a d) aerobic bacteria take up oxygen, which changes the measurement of the rate of photosynthesis. In the first lab period you will extract chlorophyll, the green pigment in leaves, from spinach in the second lab period you will investigate the interaction of light with chlorophyll read in appendix b the reference section titled electronic structure of matter copied from reference 1 before performing experiment investigating the.
The rate of photosynthesis was the greatest under white light, followed by red, blue, yellow and green, which produced no detectable photosynthesis signifying that chlorophyll reflects, rather than absorbs, green light. Abstract the ap biology students at mrhs (including this one of a kind group) conducted an experiment with spinach leaves to determine the rates of photosynthesis first, depending on the carbon dioxide available, and second, depending on the temperature. Bio 113 absorption spectrum of plant pigment lab report absorption spectrum of plant pigments that is proof that chlorophyll reflects the green wave lengths between 490 and 590 also, the best absorption of light by chlorophyll is in the red, blue and violet waves ethanol, and chlorophyll extract from spinach leaves the. Irradiation with blue, red and green monochromatic light produced fluorescence profiles that peaked 50, 100 and 150 µm, respectively, beneath the irradiated surface (vogelmann & han 2000) thus, there are interactions between leaf anatomy and the wavelength of light that determine the shape of absorption profiles.
Students conducted an experiment to determine the effect of light intensity on the rate of photosynthesis they punched 40 leaf disks from spinach leaves and used a syringe partially filled with water to pull the gases from the leaf disks so that all leaf disks sunk to the bottom of the syringe green, and red b red and yellow c blue. For this experiment, dcpip was added to cuvettes with spinach chloroplasts, which were exposed to an incandescent light at different distances for different intervals of time after each exposure, the cuvettes were placed in a spectrophotometer set at 621 nm and the absorbance of dcpip was measured. Students conducted an experiment to determine the effect of light intensity on the rate of photosynthesis they punched 40 leaf disks from spinach leaves and used a syringe partially filled with water to pull the gases from the leaf disks so that all leaf disks sunk to the bottom of the syringe.
Analysis of the absorption of green light versus red light absorption in spinach leaves 1433 words 6 pages the goal of the experiment was to determine if green light had less ability to absorb than red light in spinach leaves. The rate of photosynthesis was measured every five min under light colors of white, green, red, blue and yellow under a light intensity of 2000 lux. It is also known that green light, once absorbed by the leaves, drives photosynthesis with high efficiency (björkmann 1968, balegh and biddulph 1970, mccree 1972, inada 1976) on an absorbed quantum basis, the efficiency or photosynthetic quantum yield of green light is comparable with that of red light, and greater than that of blue light.
Production, the number and absorption wavelength preferences of the pigments involved in photosynthesis, and some possible assumptions that can be drawn from the correlation of the data gathered methods pigment samples were extracted from spinach leaves by grinding and filtering the spinach leaves taken and then suspending the pigments in acetone. Chlorophylls a and b can absorb a maximum of 600-675 and 400-475 nm ranges and will absorb light in two regions of the spectrum to test the absorption of pigments a spectrophotometer is used a graph called an absorption spectrum plots the pigment’s light absorption versus the wavelength.